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AssigneeUniversitetet I Tromsø—Norges Arktiske Universitet
InventorsMarcin PIERECHOD, Nils Peder WILLASSEN, Ulli ROTHWEILER
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The present invention relates to the use of a single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions, to dehybridize a DNA molecule or to prevent hybridisation of a complementary ssDNA, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the DNA molecule or ssDNA is present in or exposed to a solution containing one or more of the following (i) at least 350 mM of sodium ions; (ii) at least 50 mM of potassium ions; (iii) at least 150 mM of magnesium ions; or (iv) at least 200 mM of calcium ions.
Use of a single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions, to dehybridize a DNA molecule, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the DNA molecule is present in or exposed to a solution containing one or more of the following:
Use of a single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions to prevent hybridisation of complementary ssDNA, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the ssDNA is present in or exposed to a solution containing one or more of the following:
A single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions and comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical thereto, or a functional fragment thereof, and wherein the amino acid at position 17 and/or 71 has been substituted.
A nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO:4 or a fragment thereof which encodes for an SSB which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions.
A composition comprising an isolated SSB which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions and a buffer and optionally a stabilising agent, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof.
Dependencies are inferred from the claim text.