Dna random access storage system via ligation

A method of performing arbitrary, random access on a DNA pool to obtain multiple DNA strands each storing a portion of a same digital file, the method comprising:

• adding, to a vessel containing DNA strands from the DNA pool:
• a first staple that is complementary in part to a 5′-end sequence present on the multiple DNA strands and complementary in part to a first sequencing adaptor region, the first sequencing adaptor region including a first sequencing adaptor sequence and a first sequencing primer binding region,
• a second staple that is complementary in part to a 3′-end sequence present on the multiple DNA strands and complementary in part to a second sequencing adaptor region, the second sequencing adaptor region including a second sequencing adaptor sequence and a second primer binding region,
• the first sequencing adaptor region, and
• the second sequencing adaptor region;
• heating the vessel to a first temperature;
• cooling the vessel to a second temperature lower than the first temperature;
• and
• adding, to the vessel, DNA ligase to create ligation products, the ligation products include a subset of the multiple DNA strands, the subset having a sequence comprising the first sequencing adaptor region, the 5′-end sequence, a payload sequence encoding a portion of the digital file, the 3′-end sequence, and the second sequencing adaptor region.

A method of selectively preparing a DNA strand for sequencing, the method comprising:

• contacting the DNA strand with a sequencing adaptor;
• contacting the DNA strand with a staple complementary in part to an end sequence of the DNA strand and complementary in part to a first end sequence of the sequencing adaptor;
• and
• contacting the DNA strand with DNA ligase.