Antimicrobial cartridges and processes for multiplexed antimicrobial susceptibility testing
AssigneeSelux Diagnostics, Inc.
InventorsEric Stern, Kelly Flentie, Aleksandar Vacic, Frederick P. Floyd, Sarah A. Scott
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Abstract
The present invention provides, among other things, cartridges for multiplexed antimicrobial susceptibility testing (AST), single cartridges useful for both AST and quality control of AST, and systems and methods relating thereto.
Claims44
A cartridge for performing antimicrobial susceptibility testing (AST) of microorganisms derived from human samples and/or quality control (QC) microorganisms, comprising:
- (a) a first plurality of reservoirs comprising at least 8, 10, 12, 14, 16, 18, 20, 22 or 25 different antimicrobials, a plurality of antimicrobials being present at a plurality of different amounts or concentrations across different reservoirs, thereby defining a dilution series;
- and
- (c) an arrangement of a plurality of these dilution series into “QC blocks,” such that each block comprises a plurality of antimicrobials that may utilize the same QC microorganism.
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A method of performing AST or providing quality control (QC) for an AST with a cartridge comprising at least 96 or at least 384 reservoirs and at least 8 unique antimicrobials, each present in a plurality of concentrations defining a dilution series that spans both an expected clinical breakpoint range and an expected QC organism breakpoint range, the method comprising:
- inoculating the cartridge with either a patient-derived sample comprising a microorganism or at least one QC organism for an antimicrobial present on the cartridge;
- incubating the cartridge under conditions promoting microorganism growth for a period between 2 and 24 hours;
- and
- performing one or more assays to determine, for the test sample or QC organism, a minimum inhibitory concentration (MIC) for a plurality of antimicrobials on the cartridge.
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Dependencies are inferred from the claim text.