Preparation of (R)-3-Hydroxybutyric Acid or Its Salts by One-Step Fermentation
AssigneeNNB Nutrition USA, LLC
InventorsKylin Liao, Wenchao Fan
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Abstract
The subject invention relates to a process of preparing (R)-3-hydroxybutyric acid or a salt thereof by one-step fermentation with a nonpathogenic microorganism. The fermentation of (R)-3-hydroxybutyric acid was performed by supplying with certain carbon and nitrogen sources. These microorganisms include a Glutamic acid Bacterium HR057 strain or one type of genetically engineered Corynebacterium Glutamicum.
Claims18
A process for producing (R)-3-hydroxybutyric acid comprising fermenting a fermentation broth with a nonpathogenic microorganism, wherein the fermentation broth comprises carbon and nitrogen sources and an enzyme that is overexpressed by the nonpathogenic microorganism, the carbon and nitrogen sources are directly converted into (R)-3-hydroxybutyric acid by one-step fermentation with the nonpathogenic microorganism, the (R)-3-hydroxybutyric acid was recovered from fermentation broth after it was excreted during fermentation, wherein the nonpathogenic microorganism is selected from a group consisting of Corynebacterium glutamicum, Bacillus subtilis, Brevibacterium lactofermentum, Brevibacterium difficile, Brevibacterium flavum and Brevibacterium breve;
- and the nonpathogenic microorganism has the following biotransformation capability:
- converting pyruvic acid and coenzyme A to acetyl-CoA, converting acetyl-CoA into acetoacetyl-CoA, converting acetoacetyl-CoA to acetoacetic acid, and converting acetoacetic acid to (R)-3-hydroxybutyric acid.
A genetically engineered nonpathogenic microorganism selected from the group consisting of Corynebacterium glutamicum, Glutamic acid Bacterium, Bacillus subtilis, Brevibacterium lactofermentum, Brevibacterium flavum, and Brevibacterium breve,
- wherein the nonpathogentica microorganism is capable of producing and accumulating (R)-3-hydroxybutyric acid in a fermentation broth during a fermentation process;
- and
- wherein the nonpathogenic microorganism has the following biotransformation capability:
- converting pyruvic acid and coenzyme A to acetyl-CoA, converting acetyl-CoA into acetoacetyl-CoA, converting acetoacetyl-CoA to acetoacetic acid, and then converting acetoacetic acid to (R)-3-hydroxybutyric acid.
The nonpathogenic microorganism of claim 16, wherein the nonpathogenic microorganism is a Corynebacterium glutamicum strain or a Glutamic acid Bacterium strain.
The nonpathogenic microorganism of claim 17, wherein the nonpathogenic microorganism is a Corynebacterium glutamicum strain.
Dependencies are inferred from the claim text.