Engineered tyrosine ammonia lyase
AssigneeCodexis, Inc.
InventorsGjalt W. Huisman, Joyce Liu, Nikki Dellas, Chinping Chng, William Casey Hallows
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Abstract
The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
Claims62
A recombinant tyrosine ammonia lyase and/or biologically active recombinant tyrosine ammonia lyase fragment comprising an amino acid sequence comprising at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% sequence identity to SEQ ID NO:
- 2, 6, 28, 92, 308, 338, 504, and/or 736.
A recombinant tyrosine lyase variant comprising at least 90% sequence identity to at least one of the even numbered sequences in SEQ ID NOS:
- 4-780.
A recombinant tyrosine ammonia lyase comprising a sequence that is at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the polypeptide sequence of SEQ ID NO:
- 6, 28, 92, 308, 338, 504, and/or 736.
Dependencies are inferred from the claim text.