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OwnerAnthony P. Burgard, Mark J. Burk +2inventors
InventorsAnthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
Estimating the term…
The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
1-240. (canceled)
A non-naturally occurring microbial organism, comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding proteins or enzymes wherein said one or more gene disruptions confer increased production of 3-hydroxyisobutyric acid or methacrylic acid in said organism.
Dependencies are inferred from the claim text.