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US 2019/0376089 A1Published application
Type i-b crispr-cas system gene cas3-based gene editing method
AssigneeAnhui University
InventorsWangyu TONG, Yanyan TANG, Tingting XIA
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AssigneeAnhui University
InventorsWangyu TONG, Yanyan TANG, Tingting XIA
Estimating the term…
A novel gene editing system developed based on the gene cas3 in the type I-B CRISPR-Cas system in the chromosome of Streptomyces virginiae IBL14 enables the gene editing of a type I CRISPR-Cas system on biological cell genomes for the first time. In the system, Cas3 can specifically cleave a DNA fragment of interest guided by crRNA or t-DNA. The new approach can be applied to rapid, simple and correct genome editing of prokaryotic and eukaryotic cells, which provides novel supplements and selections for the commercialized gene editing system developed based on Cas9 in type H.
A type I-B CRISPR-Cas system gene cas3-based gene editing method comprising the following step:
The type I-B CRISPR-Cas system gene cas3-based gene editing method according to 2, wherein the t-DNA is a chemically and biologically synthesized t-DNA fragment with the desired genetic function, and the chemically and biologically synthesized t-DNA fragment is combined with the Cas3 expression vector or the gene editing vector, or exists independently.
Dependencies are inferred from the claim text.