Compositions and methods for mammalian genetics and uses thereof
AssigneeWhitehead Institute for Biomedical Research
InventorsThijn R. Brummelkamp, Jan E. Carette
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Abstract
The invention provides compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells. The invention further provides genes and gene products isolated using the inventive methods and methods of use thereof.
Claims22
-28. (canceled)
A mammalian cancer cell engineered to express a set of reprogramming factors sufficient to reprogram a normal mammalian somatic cell to pluripotency.
An adherent, near-haploid mammalian cell derived from a non-adherent near-haploid mammalian cell.
-49. (canceled)
A method of identifying a gene that affects cell phenotype, the method comprising the steps of:
- (a) providing a library comprising adherent near-haploid mammalian cells in culture, wherein said adherent near-haploid mammalian cells are derived from a non-adherent near-haploid mammalian cell engineered to express a set of reprogramming factors, wherein at least some of said adherent near-haploid mammalian cells comprise a nucleic acid construct integrated into their genome, and wherein the nucleic acid construct comprises a nucleic acid that allows the identification of a cell containing said nucleic acid;
- (b) identifying a cell containing said nucleic acid construct integrated into its genome, wherein the cell exhibits a phenotype of interest;
- and (c) identifying a gene into which the nucleic acid construct integrated, thereby identifying a gene that affects cell phenotype.
Dependencies are inferred from the claim text.