U6 polymerase iii promoter and methods of use
AssigneeE. I. Du Pont de Nemours and Company
InventorsZHONGSEN LI, ZHAN-BIN LIU
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Abstract
The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
Claims19
-22. (canceled)
A recombinant DNA construct comprising a promoter nucleotide sequence comprising a region of homology of a least 200 nucleotides with SEQ ID NO:
- 9, operably linked to at least one heterologous sequence, wherein said nucleotide sequence controls the expression of a heterologous sequence.
A plant transformed with a recombinant DNA construct comprising a promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter controls expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a nucleotide sequence comprising a region of homology of a least 200 nucleotides with SEQ ID NO:
- 9.
A recombinant DNA construct comprising a promoter comprising a nucleotide sequence comprising a region of homology of a least 200 nucleotides with SEQ ID NO:
- 9, wherein said promoter is operably linked to a heterologous nucleic acid fragment, and wherein said heterologous nucleic acid fragment expresses a guide RNA.
Dependencies are inferred from the claim text.