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AssigneeCargill, Incorporated
InventorsChristopher K. Miller, Ana Negrete-Raymond, Jon Veldhouse, Amit Vas
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Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.
1-52. (canceled)
An engineered host cell comprising at least first, second, third, and fourth exogenous nucleic acids each comprising a sequence encoding a common heterologous glucoamylase polypeptide, or encoding two or more different heterologous glucoamylase polypeptides having 90% or greater sequence identity to each other, wherein the first, second, third, and fourth exogenous nucleic acids have nucleic acid sequences that are different from one another.
An engineered host cell comprising two or more exogenous nucleic acids each comprising a sequence encoding a common heterologous glucoamylase polypeptide, or encoding two or more different glucoamylase polypeptides having 90% or greater sequence identity to each other, wherein the two or more exogenous nucleic acids have nucleic acid sequences that are different from one another.
Dependencies are inferred from the claim text.