Use of microbial consortia in the production of multi-protein complexes
AssigneeThe Regents of the University of California
InventorsFernando VILLARREAL, Cheemeng TAN
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Abstract
The present invention provides microbial cultures (referred to here as microbial consortia) comprising a plurality of microbial strains each strain comprising a different recombinant plasmid including a gene encoding a different protein involved in translation of mRNA. The protein expression level of each protein is controlled to a pre-defined level, such that the proteins are capable of forming a multi-protein complex which translates an mRNA molecule into a polypeptide in a reaction mixture.
Claims22
A microbial culture comprising a plurality of microbial strains, each strain comprising a different recombinant plasmid including a gene encoding a different protein involved in translation of mRNA, wherein the protein expression level of each protein is controlled to a pre-defined level, such that the proteins are capable of forming a multi-protein complex which translates an mRNA molecule into a polypeptide in a reaction mixture.
(canceled)
A method of making a multi-protein complex which is capable of translating an mRNA molecule into a polypeptide in a reaction mixture, the method comprising:
- (a) providing a microbial culture comprising a plurality of microbial strains, each strain comprising a different recombinant plasmid including a gene encoding a different protein involved in translation of mRNA, wherein the protein expression level of each protein is controlled to a pre-defined level, such that the proteins are capable of forming a multi-protein complex;
- and
- (b) simultaneously isolating the proteins from the microbial culture, thereby forming the multi-protein complex.
A method of translating an mRNA molecule into a polypeptide, the method comprising:
- (a) providing a microbial culture comprising a plurality of microbial strains, each strain comprising a different recombinant plasmid comprising a gene encoding a different protein involved in translation of mRNA, wherein the protein expression level of each protein is controlled to a pre-defined level, such that the proteins are capable of forming a multi-protein complex which translates an mRNA molecule into a polypeptide in a reaction mixture;
- (b) simultaneously isolating the proteins from the microbial culture, thereby forming the multi-protein complex;
- (c) forming a reaction mixture comprising the multi-protein complex, amino acids, ribosomes, and the mRNA molecule or a DNA molecule encoding the mRNA;
- (d) incubating the reaction mixture under conditions suitable for translation of the mRNA molecule into a polypeptide;
- and
- (e) isolating the polypeptide.
(canceled)
Dependencies are inferred from the claim text.