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US 2019/0376068 A1Published application
Promoter construct for cell-free protein synthesis
AssigneeFraunhfer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
InventorsMatthias Buntru, Simon Vogel, Stefan Schillberg
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Abstract
The technology provided herein relates to novel promoter constructs, method and systems for an increase in the target protein yield and/or allowing the use of lower template concentration in the cell-free protein synthesis systems.
Claims11
A DNA promoter construct suitable for an efficient cell-free protein synthesis of a target polypeptide, wherein said DNA promoter construct comprises in a 5′-3′ direction:
- a) a promoter element of an RNA polymerase;
- b) an enhancing element of at least 6 consecutive nucleotides including the transcription start site, wherein the enhancing element comprises the sequence GGGAGA, GCAAGA or GGAAGA;
- c) a 5′ UTR sequence element;
- and
- d) a translation initiation start site.
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Dependencies are inferred from the claim text.