Thermostable reverse transcriptase
AssigneeBoard of Regents, The University of Texas System
InventorsAndrew ELLINGTON, Jared ELLEFSON, Jimmy GOLLIHAR
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Abstract
Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate.
Claims23
1-84. (canceled)
A recombinant Archaeal Family-B polymerase comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1 and comprising an amino acid substitution corresponding to positions R97, Y384, V389, Y493, F587, E664, G711, and W768 of SEQ ID NO:
- 1.
A recombinant Archaeal Family-B polymerase comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1 and comprising an amino acid substitution corresponding to positions R97, Y384, V389, Y493, 1521, F587, E664, G711, N735, and W768 of SEQ ID NO:
- 1.
A recombinant Archaeal Family-B polymerase comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 1 and comprising an amino acid substitution corresponding to positions F38, R97, K118, M137, R381, Y384, V389, A490, K466, Y493, T514, 1521, F587, E664, G711, E734, N735, and W768 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.