Systems, methods, and apparatus for induced pluripotent stem cell isolation and combinatorial production

A method for electronically determining and compiling a list of binding agents from a storage facility comprising one or more electronic files, the method comprising:

• receiving, by a processor of a computer device, data from the detection of a plurality of cell surface markers expressed on the surface of iPSCs;
• determining, by the processor, one or more vectors contacted to the colony of cells based on the detected cell surface markers;
• mapping, by the processor, the determined one or more vectors to a list of a plurality of cell surface markers contained in the one or more files of the storage facility;
• and
• mapping one or more binding agents contained in an electronic compendium having a binding specificity for each mapped cell surface marker.

A method comprising:

• providing a heterogeneous mixture of a plurality of colonies of cells having been contacted with a plurality of vectors;
• producing a colony of iPSCs from the plurality of colonies of cells, wherein the colony of iPSCs comprises varied genetic edits;
• and
• separating the colony of iPSCs into one or more homogeneous vector lineages.

A method comprising:

• expanding a population of cells in a cell culture medium for greater than 15 days;
• following the expanding step, isolating the population of cells that express a cell surface marker of interest;
• and
• contacting a plurality of viral vectors with the enriched population of cells.