Methods for manipulating cell fate
AssigneeThe McLean Hospital Corporation
InventorKwang-Soo Kim
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Abstract
Disclosed herein are methods of generating induced pluripotent stem cells. The method involves providing a somatic or non-embryonic cell population, contacting the somatic or non-embryonic cell population with a quantity of at least one reprogramming factor, an agent that downmodulates SIRT2, and/or an agent that upmodulates SIRT1, and culturing the somatic or non-embryonic cells for a period of time sufficient to generate at least one induced pluripotent stem cell. Methods for differentiating a cell by upmodulating SIRT2 and/or downmodulating SIRT1 are also provided herein. Also disclosed are cell lines and pharmaceutical compositions generated by use of the methods.
Claims27
1) A method to generate induced human pluripotent stem cells comprising delivering to a somatic or non-embryonic cell population an effective amount of one or more reprogramming factors and also an agent that downmodulates SIRT2, and culturing the somatic or non-embryonic cell population for a period of time sufficient to generate at least one induced human pluripotent stem cell.
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22) A method to generate differentiated cells comprising delivering to a pluripotent cell population an agent that upmodulates SIRT2 and culturing the population under differentiating conditions for a period of time sufficient to generate at least one differentiated cell.
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Dependencies are inferred from the claim text.