PREPARATION OF FETAL NUCLEATED RED BLOOD CELLS (NRBCs) FOR DIAGNOSTIC TESTING
AssigneeKellbenx Incorporated
InventorsHassan BENNANI, Leonard H. KELLNER
Life of this patent
Estimating the term…
Abstract
The disclosure relates to methods of preparation of fetal nucleated red blood cells (NRBCs) from biological samples for diagnostic testing.
Claims30
A method of enriching for fetal nucleated red blood cells (fNRBCs) from a biological sample, comprising:
- (a) subjecting the biological sample to density separation to obtain a fNRBC-containing cell fraction;
- (b) subjecting the fNRBC-containing cell fraction obtained in step (a) to magnetic activated cell sorting (MACS) using at least one fNRBC positive selection reagent to obtain a MACS-sorted cell population;
- (c) fluorescently labeling cells in the MACS-sorted cell population obtained in step (b) with at least one fNRBC positive selection reagent to obtain a fluorescently labeled cell population;
- and
- (d) sorting the fluorescently labeled cell population obtained in step (c) by flow cytometry to select for fNRBCs, thereby obtaining a population of cells enriched for fNRBCs.
2-3. (canceled)
5-9. (canceled)
(canceled)
A method of enriching for fNRBCs from a biological sample, comprising:
- (a) subjecting the biological sample to density separation to obtain a fNRBC-containing cell fraction;
- (b) subjecting the fNRBC-containing cell fraction obtained in step (a) to MACS using at least two fNRBC positive selection reagents to obtain a MACS-sorted cell population;
- (c) performing micromanipulation on the MACS-sorted cell population obtained in step (b) to isolate individual fNRBCs or groups of fNRBCs.
(canceled)
(canceled)
24-25. (canceled)
A FACS-sorted cell population containing (a) at least 2, at least 5 or at least 10 and/or (b) up to 15, up to 25, or up to 35 fNRBCs enriched from maternal blood.
29-30. (canceled)
(canceled)
35-38. (canceled)
40-42. (canceled)
Dependencies are inferred from the claim text.