Definitive endoderm
AssigneeViaCyte, Inc.
InventorsKevin Allen D'Amour, Alan D. Agulnick, Emmanuel E. Baetge
Life of this patent
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Abstract
Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.
Claims20
An in vitro method of making a cell population comprising definitive endoderm cells, the method comprising:
- (a) obtaining a cell population comprising human pluripotent stem cells in medium comprising no serum or serum replacement;
- and
- (b) providing the cell population comprising human pluripotent stem cells with a TGFβ super family member,
- thereby producing a cell population comprising definitive endoderm cells.
An in vitro method of making a cell population comprising definitive endoderm cells comprising:
- (a) obtaining a cell population comprising human pluripotent stem cells in a medium comprising less than about 10% serum;
- and
- (b) providing the cell population with a TGFβ super family member,
- thereby producing a cell population comprising definitive endoderm cells.
A method of differentiating human pluripotent cells, said method comprising culturing a population of pluripotent human cells in a medium comprising no serum or serum replacement and a TGFβ superfamily growth factor thereby differentiating the human pluripotent cells.
Dependencies are inferred from the claim text.