Novel nucleoside triphosphate transporter and uses thereof
AssigneeThe Scripps Research Institute
InventorsFloyd E. ROMESBERG, Yorke ZHANG
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Abstract
Disclosed herein are proteins, methods, cells, engineered microorganisms, and kits for generating a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum. Also disclosed herein proteins, methods, cells, engineered microorganisms, and kits for production of a nucleic acid molecule that comprises an unnatural nucleotide utilizing a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum.
Claims29
A method of increasing production of a nucleic acid molecule in a cell, comprising:
- (a) expressing in the cell a recombinant nucleoside triphosphate transporter, wherein the recombinant nucleoside triphosphate transporter comprises an amino acid sequence comprising a deletion,
- (b) incubating the cell with one or more unnatural nucleoside triphosphates, and
- (c) transporting the one or more unnatural nucleoside triphosphates into the cell using the recombinant nucleoside triphosphate transporter.
A method of increasing production of a DNA nucleic acid molecule in a cell comprising:
- (a) expressing in the cell a recombinant nucleoside triphosphate transporter, wherein the recombinant nucleoside triphosphate transporter comprises an amino acid sequence comprising a deletion,
- (b) incubating the cell with one or more unnatural 2′-deoxyribonucleoside triphosphates, and
- (c) transporting the one or more unnatural 2′-deoxyribonucleoside triphosphates into the cell using the recombinant nucleoside triphosphate transporter, wherein the one or more unnatural 2′-deoxyribonucleoside triphosphates comprise a base selected from the group consisting of:
A method of increasing production of a RNA nucleic acid molecule in a cell, comprising:
- (a) expressing in the cell a recombinant nucleoside triphosphate transporter, wherein the recombinant nucleoside triphosphate transporter comprises an amino acid sequence comprising a deletion,
- (b) incubating the cell with one or more unnatural ribonucleoside triphosphates, and
- (c) transporting the one or more unnatural ribonucleoside triphosphates into the cell using the recombinant nucleoside triphosphate transporter, wherein the one or more unnatural ribonucleoside triphosphates comprise a base selected from the group consisting of:
Dependencies are inferred from the claim text.