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US 2019/0375763 A1Published application
Reagents for reversibly protecting biological molecules
AssigneesBiomerieux, Universite De Caen Normandie
InventorsSylvain URSUEGUI, Alain LAURENT, Ali LAAYOUN, Frédéric FABIS
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Abstract
The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.
Claims17
Compound of the following formula (I):
- wherein
- X is a covalent bond or a C1-C4 alkyl,
- Y is a nucleophilic group,
- Z1, Z2, Z3 each represent, independently of one another, N or C,
- R1 is H, a substituted or unsubstituted C1-C6 alkyl group, a substituted or unsubstituted alkenyl, a substituted or unsubstituted aryl group, or a substituted or unsubstituted heterocycle,
- R2 is a thermolabile and/or acid-labile protecting group,
- R3 is H, a substituted or unsubstituted C1-C12 alkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heterocycle, an acyl group, a substituted or unsubstituted alkenyl group, a halogen, or a cyano group.
4 more elements
Protected biological molecule of the following formula (III):
- wherein
- Biomol is a biological molecule
- W is a nucleophilic group of the biological molecule,
- X is a covalent bond or a C1-C4 alkyl,
- Y is a nucleophilic radical,
- Z1, Z2, Z3 each represent, independently of one another, N or C,
- R1 is H, a substituted or unsubstituted C1-C6 alkyl group, a substituted or unsubstituted alkenyl, a substituted or unsubstituted aryl group, or a substituted or unsubstituted heterocycle,
- R2 is H or a thermolabile and/or acid-labile protecting group,
- R3 is H, a substituted or unsubstituted C1-C12 alkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heterocycle, an acyl group, a substituted or unsubstituted alkenyl group, a halogen, or a cyano group.
6 more elements
Dependencies are inferred from the claim text.