Proteinic biomass preparation comprising a non-native organism of the clostridia class
AssigneeWhite Dog Labs, Inc.
InventorsBryan P. TRACY, Shawn William JONES, Aharon M. EYAL
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Abstract
A proteinic biomass preparation comprising a non-native organism of the Clostridia class, which organism expresses (i) a modified aspartate kinase; (ii) a modified homoserine dehydrogenase; (iii) a modified homoserine kinase; (iv) a modified anthranilate synthase; (v) a functional lycopene pathway and the genes crtY, crtW, and crtZ; and/or (vi) a functional oleic acid pathway and the four gene operon (pfaABCD). Methods of producing proteinic biomass preparations are also described.
Claims30
A proteinic biomass preparation comprising a non-native organism of the Clostridia class, which organism expresses
- (i) a modified aspartate kinase characterized by reduced lysine inhibition, reduced threonine inhibition, and/or reduced methionine inhibition compared with the unmodified enzyme in native organism of the same genus and species;
- (ii) a modified homoserine dehydrogenase characterized by reduced threonine inhibition compared with the unmodified enzyme in native organism of the same genus and species;
- (iii) a modified homoserine kinase characterized by reduced methionine inhibition compared with the unmodified enzyme in native organism of the same genus and species;
- (iv) a modified anthranilate synthase characterized by reduced tryptophan inhibition compared with the unmodified enzyme in native organism of the same genus and species;
- (v) a functional lycopene pathway and the genes crtY, crtW, and crtZ and/or
- (vi) a functional oleic acid pathway and the four gene operon (pfaABCD).
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A proteinic biomass preparation comprising an organism of the Clostridia class, wherein said preparation comprises,
- (i) on dry basis at least 55% wt protein;
- (ii) on total protein content, at least 6% wt lysine;
- (iii) on total protein content, at least 3% wt threonine;
- (iv) on total protein content, at least 1.5% wt methionine;
- (v) on total on total protein content, at least 0.5% wt tryptophan;
- (vi) on a dry basis, at least 0.01% wt astaxanthin;
- (vii) on a dry basis, at least 0.1wt eicosapentaenoic acid, and/or
- (viii) on a dry basis, at least 0.1% wt docosahexaenoic acid.
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Dependencies are inferred from the claim text.