System and methods for bilirubin analysis

A method for measuring bilirubin levels in a subject, the method comprising:

• contacting a blood sample from the subject and a release agent, wherein the blood sample comprises bilirubin bound to albumin and the release agent is configured to release the bound bilirubin from the albumin;
• measuring electrochemical data of the blood sample using an electrochemical cell;
• and
• determining concentrations of conjugated bilirubin, unconjugated bilirubin, and total serum bilirubin using the electrochemical data.

A method for measuring bilirubin levels in a subject, the method comprising:

• providing a blood sample to be measured from the subject, wherein the blood sample comprises bilirubin bound to albumin;
• contacting the blood sample and a release agent, the release agent configured to release the bound bilirubin from the albumin;
• measuring electrochemical data of the blood sample using an electrochemical cell;
• and
• determining concentrations of conjugated bilirubin, unconjugated bilirubin, total serum bilirubin, and albumin using the electrochemical data.