Glycan analysis method
AssigneeShimadzu Corporation
InventorTakashi NISHIKAZE
Life of this patent
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Abstract
Glycans having a branched structure are labeled with a labeling agent, such as 2-aminobenzoic acid, having one site that is easily negatively charged. At this time, reduction which is usually performed in labeling by reduction amination is not performed. A sample for mass spectrometry containing the labeled form of glycans thus obtained is prepared, and is subjected to MS/MS analysis in negative ion mode. In the MS/MS spectra obtained by the MS/MS analysis, peaks of E ions, D ions and the like which reflect the branched structure clearly appear. As a result, structural analysis of an entirety of the glycan including the branched structure can be easily performed.
Claims10
A glycan analysis method for analyzing a structure of glycan using mass spectrometry, the glycan analysis method comprising:
- a) a sample preparation step of preparing a sample by labeling glycan to be analyzed without reduction, using a labeling agent having at least one site capable of stably presenting a negative charge in a molecule;
- and
- b) an analysis execution step of subjecting the sample to MS/MS analysis in negative ion mode,
- wherein branched structural information of the glycan is acquired from a result of MS/MS analysis.
Dependencies are inferred from the claim text.