Method of detecting cleaved snap25 in tissue samples
AssigneeAllergan, Inc.
InventorsRon S. Broide, Brian Cai, Ester Fernandez-Salas, Joseph Francis, Catherine Rheaume
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Abstract
Methods and compositions for detecting BoNT/A enzymatic activity in tissues or a tissue sample are described herein. The invention encompasses antibodies that bind preferentially to BoNT/A cleaved SNAP25 and is able to preferentially detect BoNT/A cleaved SNAP25, as compared to intact (non-cleaved) SNAP25, in a tissue sample.
Claims18
A method of diagnosing if a tissue has been exposed to BoNT/A enzymatic activity comprising contacting a tissue sample suspected of having been exposed to BoNT/A enzymatic activity with an anti-SNAP25 antibody wherein the antibody binds preferentially to BoNT/A cleaved SNAP25;
- and detecting whether the anti-SNAP25 antibody bound to the tissue sample, wherein the presence of the anti-SNAP25 antibody binding to the tissue sample indicates that the tissue sample has been exposed to BoNT/A activity.
A kit for diagnosing if a tissue has been exposed to BoNT/A enzymatic activity comprising an anti-SNAP25 antibody, wherein the antibody binds preferentially to BoNT/A cleaved SNAP25.
Dependencies are inferred from the claim text.