Zika Virus Assay Systems
AssigneeUniversity of Miami
InventorsSylvia Daunert, Sapna K. Deo, Emre Dikici, Marcello Mascini Mascini, Devon Pawley
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Abstract
Provided herein are Zika virus (ZIKV) binding constructs, e.g., antibodies and antigen-binding fragments thereof and antibody mimetics, as well as related conjugates, polypeptides, nucleic acids, expression vectors, host cells, kits, and assay systems. Methods detecting ZIKV infection and/or ZIKV exposure and/or ZIKV immunity are provided.
Claims38
An assay system comprising a porous matrix comprising at least three zones, Zone A, Zone B, and Zone C, wherein
- Zone A comprises a binding construct that binds to a Zika virus (ZIKV) protein and does not bind to a Dengue virus (DENV) protein, wherein the binding construct is not bound to a Zika virus,
- Zone B comprises a binding construct that binds to a ZIKV protein and does not bind to a DENV protein, wherein the binding construct is bound to a Zika virus, and
- Zone C comprises a secondary antibody which binds the binding construct of Zone A and Zone B.
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A binding construct comprising the structure A1-B-A2 wherein A1 is a first peptide arm that binds to a first binding site of a target protein, A2 is a second peptide arm that binds to a second binding site of the target protein, and B is a bridge peptide which links A1 to A2, optionally, wherein the bridge peptide is about 5 to about 10 amino acids in length.
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Dependencies are inferred from the claim text.