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US 2019/0317080 A1Published application
Kits for labeling of biomarkers and methods of using the same
AssigneeRareCyte, Inc.
InventorsDaniel E. CAMPTON, Yao SUN
Life of this patent
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Abstract
Kits, compositions, and methods for labeling target materials or target analytes are considered. The composition can include one or more affinity molecules indirectly conjugated to one or more detection moieties. The kit can be used to detect a single biomarker or multiple biomarkers. The kit can be used to perform a single round of labeling or multiple rounds of labeling.
Claims30
A kit comprising:
- a first affinity molecule-first oligonucleotide conjugate;
- a horseradish peroxidase (“HRP”) molecule-second oligonucleotide conjugate;
- and
- a tyramide-first detection moiety conjugate,
- wherein the first and second oligonucleotides are complementary.
2 more elements
A method for labeling a target analyte of a sample, the method comprising:
- adding, to the sample, a first affinity molecule-first oligonucleotide conjugate;
- adding, to the sample, a horseradish peroxidase (“HRP”) molecule-second oligonucleotide conjugate;
- and
- adding, to the sample, a tyramide-first detection moiety conjugate,
- wherein the first and second oligonucleotides are complementary and hybridize with each other.
2 more elements
Dependencies are inferred from the claim text.