Transposition of Native Chromatin for Personal Epigenomics

A composition comprising:

• a permeabilized cell nucleus comprising a plurality of polynucleotide molecules;
• an insertional enzyme complex;
• and
• an insert element comprising at least one nucleic acid comprising a predetermined sequence.

A composition comprising:

• a permeabilized cell nucleus comprising a plurality of polynucleotide molecules, wherein the plurality of polynucleotide molecules comprises genomic DNA comprising a plurality of open chromatin regions;
• an insertional enzyme complex;
• and
• an insert element comprising at least one nucleic acid comprising a predetermined sequence.

A composition comprising:

• a permeabilized cell nucleus;
• an insertional enzyme complex comprising a transposase enzyme;
• and
• a plurality of tagged nucleic acid fragments, wherein each tagged nucleic acid fragment comprises a first sequencing adapter and a second sequencing adapter;
• wherein each tagged nucleic acid fragment is derived from a region of open chromatin.

A composition comprising:

• a permeabilized cell nucleus;
• an insertional enzyme complex comprising a transposase enzyme;
• and
• a plurality of tagged nucleic acid fragments, wherein each tagged nucleic acid fragment comprises a first sequencing adapter and a second sequencing adapter;
• wherein the insertional enzyme complex and the tagged nucleic acid fragments are bound to one or more regions of open chromatin within the cell nucleus in a substantially sequence independent manner to form a high affinity macromolecule complex.