Digital and quantitative pcr measuring method for nucleic acid sample
AssigneeQuark Biosciences Taiwan, Inc.
InventorsCheng-Wey Wei, Chang-Wei Huang
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Abstract
A digital and quantitative PCR (dqPCR) measuring method for a nucleic acid sample is provided, including providing a testing plate having a plurality of reaction wells, so as to perform a dqPCR reaction on the nucleic acid sample. Especially for the nucleic acid sample to be tested having a plurality of nucleic acid targets with different concentration ranges of wide variations, digital PCR and quantitative PCR can be performed simultaneously, so as to quantify the copy number of nucleic acid targets.
Claims8
A measuring method for a nucleic acid sample, which simultaneously performs a digital PCR and a quantitative PCR to detect the nucleic acid sample with a high-concentration nucleic acid target and a low-concentration nucleic acid target at one time, wherein a copy number of the high-concentration nucleic acid target in the nucleic acid sample is greater than 10000, and a copy number of the low-concentration nucleic acid target in the nucleic acid sample is 10000 or less.
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