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US 2019/0316178 A1Published application
Detection of hydroxymethylcytosine bases
AssigneeRamot at Tel-Aviv University Ltd.
InventorsYuval EBENSTEIN, Micha FRIDMAN
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Abstract
Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.
Claims19
A method of detecting 5-hydroxymethyl-cytosine in a DNA sample, the method comprising:
- (a) reacting the DNA sample with a 5-hydroxymethyl-cytosine-specific fluorescent labeling agent under conditions which allow staining of the DNA sample with said 5-hydroxymethyl-cytosine-specific fluorescent labeling agent so as to obtain a 5-hydroxymethyl-cytosine-labeled DNA sample;
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- (b) measuring fluorescence intensity of said 5-hydroxymethyl-cytosine-labeled DNA sample (X) and adsorption intensity of the DNA, at 260 nm (Y), wherein a ratio between X to Y is indicative of presence or level of 5-hydroxymethyl-cytosine in the DNA sample.
Dependencies are inferred from the claim text.