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US 2019/0316155 A1Published application
Compositions for linking dna-binding domains and cleavage domains
AssigneeSangamo Therapeutics, Inc.
InventorsDavid Paschon, Lei Zhang
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Abstract
Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.
Claims17
A fusion protein comprising
- a DNA-binding domain having an N-terminus and a C-terminus, wherein the DNA-binding domain binds to a nucleotide target site;
- a FokI cleavage domain having an N-terminus and a C-terminus;
- and
- a linker between the C-terminus of the DNA-binding domain and the N-terminus of the cleavage domain, wherein the linker comprises LRGSPISRARPLNPHP (SEQ ID NO:272);
- LRGSISRARPLNPHP (SEQ ID NO:273);
- LRGSPSRARPLNPHP (SEQ ID NO:274);
- LRGSSRARPLNPHP (SEQ ID NO:275);
- LRGSYAPMPPLALASP (SEQ ID NO:276);
- LRGSAPMPPLALASP (SEQ ID NO:277);
- LRGSPMPPLALASP (SEQ ID NO:278);
- or LRGSMPPLALASP (SEQ ID NO:279).
8 more elements
Dependencies are inferred from the claim text.