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US 2019/0316144 A1Published application
In vivo generation of water-soluble cannabinoids in plant cell suspension cultures
AssigneeTrait Biosciences, Inc.
InventorsRichard T. Sayre, Elton Carvalho Gonçalves, Tawanda Zidenga, Stephanie Willette
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Abstract
The present invention includes systems, methods and compositions for the in vivo bioconversion of water-soluble cannabinoids in plant cell suspension cultures.
Claims30
An in vivo method of producing water-soluble cannabinoids comprising the steps of:
- genetically modifying a tobacco cell to express:
- a nucleotide sequence encoding a heterologous cytochrome P450 operably linked to a promoter having hydroxylation or oxidation activity toward one or more cannabinoids to generate a hydroxylated cannabinoid or a cannabinoid carboxylic acid;
- a nucleotide sequence encoding a heterologous P450 oxidoreductase operably linked to a promoter that facilitates electron transfer from nicotinamide adenine dinucleotide phosphate (NADPH) to the cytochrome P450;
- wherein said tobacco cell expresses at least one endogenous glycosyltransferase protein having glycosylation activity towards one or more cannabinoids.
- establishing a suspension cell culture of said genetically modified tobacco cells;
- introducing one or more cannabinoids to said suspension cell culture of said genetically modified tobacco cells;
- chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids;
- and
- isolating said water soluble glycosylated cannabinoids.
6 more elements
An in vivo method for the generation of water-soluble cannabinoids comprising the steps of:
- establishing a suspension cell culture of tobacco cells that expresses at least one endogenous glycosyltransferase protein having glycosylation activity towards one or more cannabinoids;
- introducing one or more cannabinoids to said suspension cell culture of tobacco cells;
- and
- chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids through the action of said at least one endogenous glycosyltransferase.
1 more element
A non-genetically modified in vivo method of producing water-soluble cannabinoids comprising the steps of:
- establishing a plant cell suspension cell culture from a non-cannabinoid producing plant that expresses at least one endogenous glycosyltransferase having glycosylation activity towards one or more cannabinoids;
- introducing one or more cannabinoids to said plant cell suspension culture;
- chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids through the action of said at least one endogenous glycosyltransferase;
- isolating said glycosylated cannabinoids from said plant cell suspension culture.
1 more element
A method of producing water-soluble cannabinoids comprising the steps of:
- genetically modifying a plant cell to express a nucleotide sequence operably linked to a promoter encoding a heterologous glycosyltransferase from a tobacco plant having glycosylation activity towards one or more cannabinoids;
- establishing a suspension cell culture of said genetically modified plant cells;
- introducing one or more cannabinoids to said suspension cell culture of said genetically modified plant cells;
- chemically converting said one or more cannabinoids into one or more water soluble glycosylated cannabinoids;
- and
- isolating said glycosylated cannabinoids from said plant cell suspension culture.
3 more elements
Dependencies are inferred from the claim text.