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AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsSCOTT DIEHN, AJIT NOTT, DAVID A SELINGER, CARL SIMMONS, PRIYANKA BHYRI, VENKATA S TAVVA
Estimating the term…
The invention relates to gene expression regulatory sequences, specifically introns that act as enhancers of gene expression, the promoter and terminator sequences endogenously associated with these introns. Presence of these intronic enhancer sequences in proximity to promoter sequences leads to enhancement of gene expression. Methods of finding such new intronic enhancer sequences and using them to generate transgenic plants are also described.
A recombinant DNA construct comprising an intron operably linked to a promoter, a heterologous polynucleotide and a terminator wherein the intron comprises a nucleotide sequence that has at least 95% sequence identity to SEQ ID NO:
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A recombinant DNA construct comprising an intron operably linked to a promoter and a heterologous polynucleotide, wherein the intron comprises a nucleotide sequence that comprises at least one copy of SEQ ID NO:
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A method for identifying an intron useful for enhancing transgene expression in a monocotyledenous plant comprising the steps of:
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Dependencies are inferred from the claim text.