Oligonucleotide compositions with enhanced efficiency
AssigneeLife Technologies Corporation
InventorsTod Woolf, Margaret Taylor
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Abstract
The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. This invention also provides methods of inhibiting protein synthesis in a cell and methods of identifying oligonucleotide compositions that inhibit synthesis of a protein in a cell.
Claims23
-33. (canceled)
A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of three or more different double-stranded oligonucleotides in equimolar concentrations,
- wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene,
- each double-stranded oligonucleotide consists of two separate strands,
- each strand is between 15 and 40 nucleomonomers in length;
- wherein the combination is capable of RNA interference (RNAi).
A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of two different double-stranded oligonucleotides in equimolar concentrations,
- wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene,
- each double-stranded oligonucleotide consists of two separate strands,
- each strand is between 15 and 40 nucleomonomers in length,
- wherein the combination is capable of RNA interference (RNAi).
A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of three, four, five, six, seven or eight different double-stranded oligonucleotides in equimolar concentration,
- wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene,
- each double-stranded oligonucleotide consists of two separate strands,
- each strand is between 15 and 40 nucleomonomers in length,
- wherein the combination is capable of RNA interference (RNAi).
Dependencies are inferred from the claim text.