Back to results
US 2019/0316131 A1Published application
Methods For Preventing Titration Of Bimolecular Templated Assembly Reactions By Structurally-Determined Differential Hybridizations
AssigneeTriBiotica LLC
InventorsIan Dunn, Matthew Lawler
Life of this patent
Estimating the term…
Abstract
The present disclosure provides nucleic acid molecules, and kits comprising the same, for producing templated assembly products for a cell.
Claims20
A nucleic acid molecule comprising:
- a) a first stem portion comprising from about 10 to about 20 nucleotide bases;
- b) an anti-target loop portion comprising from about 16 to about 40 nucleotide bases and having a first end to which the first stem portion is linked, wherein the anti-target loop portion is substantially complementary to a target nucleic acid molecule;
- c) a second stem portion comprising from about 10 to about 20 nucleotide bases linked to a second end of the anti-target loop portion, wherein the first stem portion is substantially complementary to the second stem portion;
- and
- d) a reactive effector moiety linked to either the first stem portion or the second stem portion;
- wherein the Tm of the anti-target loop portion:target nucleic acid molecule is greater than the Tm of the first stem portion:second stem portion.
3 more elements
Dependencies are inferred from the claim text.