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US 2019/0316119 A1Published application
Compositions and methods for molecular labeling
AssigneeRaindance Technologies, Inc.
InventorsMichael L. Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren R. Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims15
1-31. (canceled)
A method for analyzing a nucleic acid, the method comprising:
- obtaining nucleic acid from a cell or a population of cells;
- exposing the nucleic acid to a transposase to form a reaction product;
- introducing the reaction product to a droplet comprising barcoded primers and reagents for amplification;
- conducting an amplification reaction in the droplet to produce barcoded amplicons corresponding to transposase-accessible regions of the nucleic acid;
- and
- sequencing the transposase-accessible regions.
3 more elements
Dependencies are inferred from the claim text.