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AssigneesVib Vzw, Katholieke Universiteit Leuven, KU LEUVEN R&D, GlobalYeast N.V.
InventorsJohan Thevelein, Mekonnen Demeke, Maria Remedios Foulquié Moreno, Stijn De Graeve, Edgard Valdomiro Charles Belo
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The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.
A eukaryotic microbial cell comprising a nucleotide sequence, the expression of which confers to, or increases in the cell the ability to directly isomerise xylose into xylulose, wherein the nucleotide sequence encodes a polypeptide with xylose isomerase activity, which polypeptide comprises an amino acid sequence that has at least 68% sequence identity with the amino acid sequence of SEQ ID NO. 7, and wherein preferably the nucleotide sequence encodes an amino acid sequence that is obtainable from a bacterium of the genus Eubacterium, more preferably a bacterium of the species Eubacterium sp. CAG_180.
Dependencies are inferred from the claim text.