Solubilized enzyme and uses thereof

The mixture of any of the preceding claims, further comprising cellular debris, one or more ribosomal component, one or more host protein, and/or host nucleic acid comprising DNA and/or RNA.

The mixture of any of the preceding claims, wherein the biomass-degrading activity is cellobiase activity, ligninase activity, endoglucanase activity, cellobiohydrolase activity, or xylanase activity.

The mixture of any of the preceding claims, wherein the polypeptide is partially unfolded, partially misfolded, or partially denatured.

The mixture of any of the preceding claims, wherein the polypeptide comprises a Cel3A enzyme from T. reesei, or a functional variant or fragment thereof.

The mixture any of the preceding claims, wherein the polypeptide is encoded by a nucleic acid sequence comprising at least 90% identity to SEQ ID NO:

• 2 or SEQ ID NO:
• 3.

The mixture of any of the preceding claims, wherein the polypeptide is aglycosylated.

The mixture of any of the preceding claims, further comprising at least one additional polypeptide having a biomass-degrading activity or a microorganism that produces one or more enzymes having a biomass-degrading activity.

A mixture comprising a plurality of polypeptides having an amino acid sequence with at least 90% identity to SEQ ID NO:

• 1 and a solubilizing agent, wherein the plurality of polypeptides have at least 20%-40% of the activity of the native polypeptide comprising SEQ ID NO:
• 1.

A method for producing a polypeptide having biomass-degrading activity comprising expressing the polypeptide in a cell and contacting the cell or a lysate thereof with a solubilizing agent at a concentration suitable for solubilizing the polypeptide.

A method for producing a polypeptide having biomass-degrading activity comprising providing a cell that has been genetically modified to produce at least one polypeptide having biomass-degrading activity, wherein at least a portion of said polypeptide having biomass-degrading activity is found in inclusion bodies, and contacting the cell, or a lysate thereof containing the inclusion bodies, with a solubilizing agent at a concentration suitable for solubilizing the polypeptide.