Screenable liver disease models and methods
OwnerSimon Messner, Jens M. Kelm +2inventors
InventorsSimon Messner, Jens M. Kelm, Radina Kostadinova-Angelova, Wolfgang Moritz
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Abstract
The present invention relates to an in vitro testing platform for the study of metabolic liver disease and related therapeutic strategies, composed of an artificial spheroidal microtissue comprising at least hepatocytes and hepatic stellate cells, and at least one type of hepatic inflammatory cells and further medium and reagents capable to establish and simulate different stages of metabolic liver disease and their progression and testing preventive and therapeutic strategies by pharmacological and/or dietary interventions.
Claims26
An artificial spheroidal microtissue comprising at least hepatocytes and hepatic stellate cells, and further at least one type of hepatic inflammatory cells.
The artificial spheroidal microtissue according to any one of the aforementioned claims, in which microtissue the cells are mammalian cells, preferably selected from the group consisting of
- human cells
- monkey cells
- pig cells
- canine cells, and/or
- rodent cells.
The artificial spheroidal microtissue according to any one of the aforementioned claims, wherein
- a) the microtissue comprises pro-activated stellate cells, and/or
- b) the microtissue represents a liver model at basal state.
A method of preparing a spheroidal microtissue comprising at least hepatocytes, hepatic stellate cells, hepatic inflammatory cells, and further endothelial cells, which method comprises the steps of
- a) producing or providing a microtissue comprising at least hepatocytes, hepatic stellate cells and hepatic inflammatory cells,
- b) producing or providing a suspension comprising sinusoidal endothelial cells,
- c) dispensing said suspension into wells or vessels comprising one or more microtissues of step a)
- d) incubating said wells or vessels to let sinusoidal endothelial cells adhere to the microtissues
Dependencies are inferred from the claim text.