Methods Of T-Lymphocyte Expansion

A method of expanding a population of memory-like T-lymphocytes comprising;

• providing an initial population of T-lymphocytes,
• increasing the intracellular concentration of a memory induction compound in the T-lymphocytes, and
• culturing the T-lymphocytes,
• thereby producing an expanded population of T-lymphocytes,
• wherein the memory induction compound has the formula (I);
• wherein:
• p is 0 or 1, and when p is 0, Y is —CH2— or —C═, and when p is 1, Y is selected from —CH—, CH2, —NH—, —S, and —O—;
• —R1 is —H, —(CH2)nCH3, —(CH2)nCH2CO2H, —CH2Ph or —CH2PhOCH2Ph;
• and when Y is —CH—, CH2, —NH—, —S, or —O—, X is a single bonded group selected from —H, —OH, —NH2, —SH, —(CH2)nCH3—(CH2)nCH2CO2H, —F, —Cl, —Br, and —I, or a double bonded group selected from ═O and ═S;
• and when Y is a double bonded —C═, X is —H;
• and
• each n is independently 0 to 12,
• and the mono- and diester forms thereof, such as the alkyl mono- and diester forms thereof.