Media for culturing stem cells
AssigneeTechnion Research & Development Foundation Limited
InventorsMichal AMIT, Joseph ITSKOVITZ-ELDOR
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Abstract
Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.
Claims21
A culture medium comprising an IL6RIL6 chimera and basic fibroblast growth factor (bFGF), wherein said IL6RIL6 chimera comprises amino acids 112-355 of GenBank Accession No. AAH89410 wherein said culture medium is capable of maintaining said human pluripotent stem cell in an undifferentiated state for at least 7 passages.
A cell culture comprising a human pluripotent stem cell and a culture medium, said culture medium comprising an IL6RIL6 chimera, wherein said IL6RIL6 chimera comprises amino acids 112-355 of GenBank Accession No. AAH89410, wherein said culture medium is capable of maintaining said human pluripotent stem cell in an undifferentiated state.
A method of expanding and maintaining human pluripotent stem cells in an undifferentiated state, the method comprising culturing the human pluripotent stem cells in a culture medium which comprises an IL6RIL6 chimera, wherein said IL6RIL6 chimera comprises amino acids 112-355 of GenBank Accession No. AAH89410, said culture medium is capable of maintaining the human pluripotent stem cells in an undifferentiated state, thereby expanding and maintaining the embryonic stem cells in the undifferentiated state.
Dependencies are inferred from the claim text.