Date Palm Medium Compositions and Methods
AssigneeBien-Etre Labs, LLC
InventorsAdnan Mjalli, Assel T. Issa, Salam A. Ibrahim, Lorraine M. Rusch, Ahmad Abuzuaiter
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Abstract
Disclosed are date palm compositions and methods of use of such compositions. Such compositions may be used as part of a growth medium for culture of microorganisms. In certain embodiments, date palm extracts of the disclosure are used as part a culture medium to grow Lactobacilli.
Claims24
A method of preparing a date palm extract (DPE) broth comprising:
- (i) obtaining a mass of date material;
- (ii) combining the date material with a suspension liquid to produce a slurry mixture;
- (iii) processing the slurry mixture to produce a date palm extract broth with fibers (DPEF);
- and
- (iv) separating the DPEF into the DPE broth and a mass of date palm fiber (DPF).
A method of preparing a date palm medium (DPM) for culturing microorganisms comprising:
- (i) preparing a buffer solution;
- (ii) buffering a date palm extract (DPE) broth with the buffer solution;
- and
- (iii) adding a nitrogen source from non-animal origin to the buffered DPE broth.
A method of preparing a date palm medium with fiber (DPFM) for culturing microorganisms comprising:
- (i) preparing a buffer solution;
- (ii) buffering a date palm extract broth with fibers (DPEF) with the buffer solution;
- and
- (iii) adding a nitrogen source from non-animal origin to the buffered DPEF.
A method of cultivating microorganisms comprising:
- (i) inoculating a date palm medium (DPM) with microorganisms;
- and
- (ii) incubating the microorganisms under conditions such that growth occurs.
A method of cultivating microorganisms comprising:
- (i) inoculating a date palm medium plus fiber (DPFM) with microorganisms;
- and
- (ii) incubating the microorganisms under conditions such that growth occurs.
A probiotic comprising bacteria cultivated by the steps of:
- (i) inoculating a date palm medium plus fiber (DPFM) with microorganisms;
- and (ii) incubating the microorganisms under conditions such that growth occurs.
Dependencies are inferred from the claim text.