Microfluidic system and method of use thereof
AssigneeNew York Stem Cell Foundation, Inc.
InventorsScott Noggle, Stephen Chang
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Abstract
This invention concerns an integrated microfluidic system that utilizes microfluidic chip technology to receive a patient sample including cells, expand the cells, reprogram the expanded cells and then store the reprogrammed cells in a microfluidic chip. These microfluidic chips with stored reprogrammed cells may then be used in scenarios of genetic differentiation into specific cell types. Overall this system and workflow is suitable as a hospital based device that will allow the generation of iPSCs from every isolating patient for downstream diagnostic or therapeutic use.
Claims43
A microfluidic system for processing a biological sample comprising:
- a) one or more microfluidic units operable to process the sample, the processing comprising:
- i) isolating cells from the sample;
- ii) optionally expanding the isolated cells to produce a population of expanded cells;
- iii) reprogramming the isolated cells or the expanded cells;
- and
- iv) storing cells from one or more of (i), (ii) or (iii);
- and
- b) one or more computer memory modules containing instructions for controlling one or more of processes (i)-(iv);
- and
- c) one or more computer processor modules configured to execute the instructions.
The system of claim 21, wherein the nuclear reprogramming factor is a polynucleotide, polypeptide, or small molecule.
Dependencies are inferred from the claim text.