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AssigneeWisconsin Alumni Research Foundation
InventorsChris Todd Hittinger, EmilyClare Patricia Baker
Estimating the term…
Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.
A polypeptide comprising at least 80% sequence identity to SEQ ID NO:
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A DNA construct comprising a heterologous promoter operably linked to a polypeptide having at least 95% sequence identity to SEQ ID NO:
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A yeast cell modified to increase as compared to a control yeast cell the expression or maltose/maltotriose transport activity of a maltose/maltotriose transporter polypeptide.
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A Saccharomyces eubayanus cell modified to increase as compared to a control Saccharomyces eubayanus cell the expression or maltose/maltotriose transport activity of a maltose/maltotriose transporter polypeptide at the plasma membrane of the Saccharomyces eubayanus cell.
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A method of selecting for maltotriose-utilizing yeast strains by continued growth in media containing maltotriose plus glucose, or maltose alone or maltose plus glucose.
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Dependencies are inferred from the claim text.