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AssigneeIndustry-University Cooperation Foundation Hanyang University Erica Campus
InventorsDong Wook KIM, Eun Jin KIM, Hyun Jin YU
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The present invention relates to a plasmid-based CTX phage replication system and Vibrio cholerae strain that can be infected by CTX phage and can be used for cholera toxin production. More particularly, the present invention provides a Vibrio cholera variant strain, which expresses a toxT protein in which tyrosine at position 139 is substituted by phenylalanine through the point mutation of a toxT gene using a plasmid-based CTX phage replication system, and is used as a receptor strain which can improve CTX phage infection efficiency and allows a plurality of CTX prophages to simultaneously infect the strain and to be inserted into the chromosome thereof, which the consequent provision of the effect of increasing the production yield of a cholera toxin.
A recombinant plasmid for replicating a CTX phage, comprising the genomic sequence of a CTX phage in which the full-length sequence of a ctxA gene, represented by SEQ ID NO:
A Vibrio cholerae variant strain, comprising:
A Vibrio cholerae variant strain which is infected using a Vibrio cholerae strain harboring one or more CTX prophages selected from the group consisting of CTX-1, CTX-cla, CTX-2, CTX-env and CTX-O139 to insert the genomic sequence of a CTX phage into its chromosome, and expresses a toxT protein in which Tyr at amino acid 139 is substituted with Phe through the point mutation of a toxT gene, wherein the substituted toxT protein includes the amino acid sequences of SEQ ID NO:
Dependencies are inferred from the claim text.