Compositions for in vivo Expression of Therapeutic Sequences in the Microbiome
AssigneeSynPhaGen LLC
InventorsTodd Bernard Parsley, Christian Furlan Freguia
Life of this patent
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Abstract
Compositions for a phage particle are disclosed. The phage particle is non-replicating and includes at least one heterologous nucleic acid sequence that is capable of being expressed in a target bacteria. The expressed heterologous nucleic acid sequence is non-lethal to the target bacteria.
Claims12
1-10. (canceled)
The phage particle according to claim 26, wherein the nucleic acid sequence encodes a regulatory RNA sequence such as, but not limited to, shRNA, siRNA, or microRNA.
12-15. (canceled)
The phage particle according to claim 26, wherein the nucleic acid sequence encodes a product capable of remediation of a toxic product in an environment.
The application of the phage particle according to claim 26, wherein the phage particle is applied to waste water or industrial waste or byproduct to decontaminate or detoxify the waste, wherein the phage particle may be co-administered with the target bacteria.
The phage particle according to claim 26, wherein the nucleic acid sequence encodes a product or products capable of metabolism of precursor material to an industrial product.
The application of the phage particle according to claim 26, wherein the phage particle is applied to industrial or environmental material to produce or improve on the production a metabolic product.
The phage particle according to claim 26, wherein the nucleic acid sequence encodes at least one product capable of conferring resistance to a pathogen.
The application of the phage particle according to claim 26, wherein the phage particle is applied to plants or soil for the treatment of a plant pathogen or pest.
The application of the phage particle according to claim 26, wherein the phage particle is applied indirectly or directly to an insect capable of transmission of a pathogen, wherein the phage particle contains at least one gene encoding a product that disrupts a replication cycle of an infectious agent within the insect.
23-25. (canceled)
A phage particle comprising:
- a phagemid including at least one nucleic acid sequence,
- an origin of replication necessary for replication of the phagemid in target bacteria and
- a receptor binding protein designed to attach, in vivo, to the target bacteria in a non-human microbiome,
- wherein the at least one nucleic acid sequence is expressed in the target bacteria and wherein the expression of the at least one nucleic acid sequence is non-lethal to the target bacteria and
- wherein a functional gene of the phage particle that sustains phage replication is silenced so that the phage particle is incapable of independent replication.
Dependencies are inferred from the claim text.