Microfluidic Device and Method for Analysing Nucleic Acids
AssigneeRobert Bosch GmbH
InventorsBernd Faltin, Jochen Rupp, Juergen Steigert, Christian Dorrer, Karsten Seidl
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Abstract
A microfluidic device for analysing nucleic acids includes a pump unit with a pumping volume, a filter unit for receiving a lysate, and a reaction chamber. The pump unit, the filter unit and the reaction chamber are arranged in the stated order in a pump direction of the pump unit. The microfluidic device is configured to pump an elution medium via the pump unit into the filter unit for elution and subsequently into the reaction chamber for further treatment.
Claims9
A microfluidic device for analyzing nucleic acids, comprising:
- a pump unit having a pump volume;
- a filter unit configured to receive a lysate;
- and
- a reaction chamber, wherein:
- the pump unit, the filter unit and the reaction chamber are arranged in the order specified in a pump direction of the pump unit, and
- the microfluidic device is configured to pump an elution medium through the pump unit for elution into the filter unit and subsequently to pump it for further processing into the reaction chamber.
A method for analyzing nucleic acids with a microfluidic device, the method comprising:
- providing a lysate of a sample;
- binding the lysate onto a filter material;
- eluting the lysate by pumping an elution medium through the filter material;
- and
- processing nucleic acids extracted in the elution medium by using the elution medium.
Dependencies are inferred from the claim text.