US 2019/0314814 A1Published application

Dna extraction device

AssigneeRegents of the University of Minnesota

InventorsKevin David Dorfman, Paridhi Agrawal

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Abstract

A DNA extraction device may include a substrate, at least one first side channel electrode, at least one second side channel electrode, optionally, at least one elongate central channel electrode, and a voltage source connected between the electrodes. The substrate defines an elongate central channel defining a major axis. A width of the elongate central channel is greater than its depth, and its depth is less than about 15 times a diameter of a cell to be introduced in the elongate central channel. The substrate also defines first and second side channels adjacent to the elongate central channel on opposite sides of the major axis. The substrate further defines first and second trapezoidally shaped connecting channels connecting the elongate central channel and the first and second side channels, respectively. The smaller parallel sides of the first and second trapezoidally shaped connecting channels open to the respective side channels.

Claims22

Dependent claims

A DNA extraction device comprising:

a substrate defining:
an elongate central channel defining a major axis, wherein a width of the elongate central channel measured perpendicular to the major axis and in a major plane of the substrate is greater than a depth of the elongate central channel measured perpendicular to the major axis and perpendicular to the major plane of the substrate, and wherein a depth of the elongate central channel is less than about 15 times a diameter of a cell to be introduced in the elongate central channel;
a first side channel adjacent to the elongate central channel on a first side of the major axis;
a second side channel adjacent to the elongate central channel on a second side of the major axis, wherein the second side is opposite the first side;
a first trapezoidally shaped connecting channel connecting the first side channel and the elongate central channel, wherein a smaller parallel side of the first trapezoidally shaped connecting channel open to the first side channel;
a second trapezoidally shaped connecting channel connecting the second side channel and the elongate central channel, wherein a smaller parallel side of the second trapezoidally shaped connecting channel open to the second side channel;
at least one first side channel electrode;
at least one second side channel electrode;
and
a voltage source connected between the at least one first side channel electrode and the at least one second side channel electrode.

7 more elements

A method comprising:

introducing a gel and a cell sample in an elongate central channel of a DNA extraction device, wherein the DNA extraction device comprises:
the elongate central channel defining a major axis, wherein a width of the elongate central channel measured perpendicular to the major axis and in a major plane of the substrate is greater than a depth of the elongate central channel measured perpendicular to the major axis and perpendicular to the major plane of the substrate, and wherein a depth of the elongate central channel is less than about 15 times a diameter of a cell to be introduced in the elongate central channel;
a first side channel adjacent to the elongate central channel on a first side of the major axis;
a second side channel adjacent to the elongate central channel on a second side of the major axis, wherein the second side is opposite the first side;
a first trapezoidally shaped connecting channel connecting the first side channel and the elongate central channel, wherein a smaller parallel side of the first trapezoidally shaped connecting channel open to the first side channel;
a second trapezoidally shaped connecting channel connecting the second side channel and the elongate central channel, wherein a smaller parallel side of the second trapezoidally shaped connecting channel open to the second side channel;
introducing a cell lysis solution to at least one of the first side channel or the second side channel to cause the cell lysis solution to enter into the elongate central channel and lyse cells in the cell sample;
introducing an electrophoresis buffer solution to at least one of the first side channel or the second side channel to cause the electrophoresis buffer solution to enter into the elongate central channel;
placing at least one first side channel electrode in the first side channel and at least one second side channel electrode in the second side channel;
applying a voltage between the at least one first side channel electrode and the at least one second side channel electrode to accumulate DNA from the cell sample into at least one of the first side channel or the second side channel.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/383,129Filed 12 Apr 2019
Priority: 13 Apr 2018
Published: 17 Oct 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

B01L 3/502715
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
C12N 15/101
By means of a solid support carrier, e.g. particles, polymers: by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…

Family

Application62/657,395Us provisional application · filed 13 Apr 2018
US 11,207,679 B2Related patent
US 2019/0314814 A1This patent

From the US filing history. Foreign family members aren’t listed.

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