Vaccines against infectious diseases caused by positive stranded rna viruses
AssigneeMedigen, Inc.
InventorsPeter Pushko, Irina Tretyakova
Life of this patent
Estimating the term…
Abstract
Compositions for protecting subjects from diseases caused by (+) SS RNA virus are described herein. The compositions include (i) a vector containing a DNA encoding a RNA molecule of an infectious (+) SS RNA virus operably linked to a eukaryotic RNA polymerase promoter and a carrier; or (ii) (+) SS RNA viruses obtained from eukaryotic cells transfected with the vector of (i) and a carrier.
Claims35
A vector comprising a DNA encoding a RNA molecule operably linked to a promoter suitable for expression of the DNA in a eukaryotic cell, wherein the RNA molecule encodes an infectious positive single stranded ((+)SS) RNA virus, and wherein the DNA comprises at least three introns.
A vector comprising a DNA encoding a chimeric RNA molecule operably linked to a promoter suitable for expression of the DNA in a eukaryotic cell, wherein the chimeric RNA molecule encodes an infectious (+)SS RNA virus, and wherein the DNA comprises at least three introns.
A method of preparing a stable plasmid, wherein the method comprises a DNA encoding a genomic RNA of an infectious (+) SS RNA virus, wherein the method comprises introducing at least three introns into the DNA, and wherein at least one intron is in the region encoding a structural protein of the (+) SS RNA virus and at least one intron is in the region encoding a non-structural protein of the (+) SS RNA virus.
Dependencies are inferred from the claim text.