Vaccine candidates against johne's disease

A method of treating or preventing Johne's disease, comprising administering to a mammal a vaccine composition comprising:

• a) an antigen selected from the group comprising MAP-1 (SEQ ID NO:21), MAP-2 (SEQ ID NO:22), MAP-3 (SEQ ID NO:23), MAP-4 (SEQ ID NO:24), MAP-5 (SEQ ID NO:25), MAP-6 (SEQ ID NO:26), MAP-7 (SEQ ID NO:27), MAP-8 (SEQ ID NO:28), MAP-9 (SEQ ID NO:29), MAP-10 (SEQ ID NO:30), MAP-11 (SEQ ID NO:31), MAP-12 (SEQ ID NO:32), MAP-13 (SEQ ID NO:33), MAP-14 (SEQ ID NO:34), MAP-15 (SEQ ID NO:35), MAP-16 (SEQ ID NO:36), MAP-17 (SEQ ID NO:37), or MAP-18 (SEQ ID NO:38) genomic islands of M. paratuberculosis or their homologs, wherein the homolog has 90% sequence identity to the selected gene;
• and
• b) a pharmaceutically acceptable carrier.

A method of treating or preventing Johne's disease, comprising administering to a mammal an immunological composition against M. paratuberculosis comprising:

• a) a eukaryotic expression vector comprising a nucleotide sequence encoding an antigen selected from the group comprising gcpE (SEQ ID NO:7), pstA (SEQ ID NO:8), kdpC (SEQ ID NO:9), papA2 (SEQ ID NO:10), impA (SEQ ID NO:11), umaA1 (SEQ ID NO:12), fabG2_2 (SEQ ID NO:13), aceAB (SEQ ID NO:14), mbtH2 (SEQ ID NO:15), lpqP (SEQ ID NO:16), map0834c (SEQ ID NO:17), cspB (SEQ ID NO:18), lipN (SEQ ID NO:19), and map1634 (SEQ ID NO:20) genes of M. paratuberculosis or their homologs, wherein the homolog has 90% sequence identity to the selected gene;
• and
• b) a pharmaceutically acceptable carrier.

A method of treating or preventing Johne's disease, comprising administering to a mammal an immunological composition against M. paratuberculosis comprising:

• a) a eukaryotic expression vector comprising a nucleotide sequence encoding an antigen selected from the group comprising MAP-1 (SEQ ID NO:21), MAP-2 (SEQ ID NO:22), MAP-3 (SEQ ID NO:23), MAP-4 (SEQ ID NO:24), MAP-5 (SEQ ID NO:25), MAP-6 (SEQ ID NO:26), MAP-7 (SEQ ID NO:27), MAP-8 (SEQ ID NO:28), MAP-9 (SEQ ID NO:29), MAP-10 (SEQ ID NO:30), MAP-11 (SEQ ID NO:31), MAP-12 (SEQ ID NO:32), MAP-13 (SEQ ID NO:33), MAP-14 (SEQ ID NO:34), MAP-15 (SEQ ID NO:35), MAP-16 (SEQ ID NO:36), MAP-17 (SEQ ID NO:37), and MAP-18 (SEQ ID NO:38) genomic islands of M. paratuberculosis or their homologs, wherein the homolog has 90% sequence identity to the selected gene;
• and
• b) a pharmaceutically acceptable carrier.

A method of treating or preventing Johne's disease, comprising administering to a mammal a composition comprising an isolated mutant M. paratuberculosis or M. avium bacterium, wherein the mutant bacterium comprises a disruption of function of a gene selected from the group consisting of gcpE (SEQ ID NO:7), pstA (SEQ ID NO:8), kdpC (SEQ ID NO:9), papA2 (SEQ ID NO:10), impA (SEQ ID NO:11), umaA1 (SEQ ID NO:12), fabG2_2 (SEQ ID NO:13), aceAB (SEQ ID NO:14), mbtH2 (SEQ ID NO:15), lpqP (SEQ ID NO:16), map0834c (SEQ ID NO:17), cspB (SEQ ID NO:18), lipN (SEQ ID NO:19), and map1634 (SEQ ID NO:20) genes of M. paratuberculosis or their homologs, wherein the homolog has 90% sequence identity to the selected gene.

A method of treating or preventing Johne's disease, comprising administering to a mammal a composition comprising an isolated mutant M. paratuberculosis or M. avium bacterium, wherein the mutant bacterium comprises a disruption of function of at least one gene from a genomic island selected from the group consisting of MAP-1 (SEQ ID NO:21), MAP-2 (SEQ ID NO:22), MAP-3 (SEQ ID NO:23), MAP-4 (SEQ ID NO:24), MAP-5 (SEQ ID NO:25), MAP-6 (SEQ ID NO:26), MAP-7 (SEQ ID NO:27), MAP-8 (SEQ ID NO:28), MAP-9 (SEQ ID NO:29), MAP-10 (SEQ ID NO:30), MAP-11 (SEQ ID NO:31), MAP-12 (SEQ ID NO:32), MAP-13 (SEQ ID NO:33), MAP-14 (SEQ ID NO:34), MAP-15 (SEQ ID NO:35), MAP-16 (SEQ ID NO:36), MAP-17 (SEQ ID NO:37), and MAP-18 (SEQ ID NO:38) genomic islands of M. paratuberculosis or their homologs, wherein the homolog has 90% sequence identity to the selected gene.