Method of Discovery of Potential Bioactive Compounds in Herbals by Regrouping after Chromatography

A method of identifying potential bioactive compounds of herbals, including the steps of:

• selecting an herbal or mixed herbal based on previous experience;
• extracting compounds from the herbals, collecting the solution produced and concentrating it;
• performing chromatography on the concentrated solution to separate the extracted compounds;
• collecting output containing separated compounds in a series of sample tubes and combining selected samples to produce a plurality of portions, each portion containing a plurality (group) of compounds;
• testing the portions for a desired bioactivity in an animal or other biological model and selecting only those portions that contain potential bioactive compounds (PBCs) for further testing;
• re-grouping the compounds represented in the portions which tested positive for bioactivity and repeating the test and selection on the new groups;
• repeating the re-grouping, test and selection steps as necessary to identify a group of compounds that contains the PBC or PBCs;
• chemically analyzing the identified PBC or PBCs.