Placental mesenchymal stem cells, methods of production, and placental mesenchymal stem cell based therapeutics
AssigneeBoard of Supervisors of Louisiana State University and Agricultural and Mechanical College
InventorYuping WANG
Life of this patent
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Abstract
A replicating in vitro cell culture of human placental mesenchymal stem cells comprising; cells which maintain a potential to differentiate to derivatives of multiple progenitor cell types throughout the culture. The progenitor cell types may include neuronal cells, oligodendrocytes and beta-cells. A method of treating a disease, disorder, or condition in a human comprising administering a pharmacologically effective dose of human placental mesenchymal stem cells to the human. A method for producing human placental mesenchymal stem cells.
Claims20
A replicating in vitro cell culture of human placental mesenchymal stem cells comprising;
- cells which maintain a potential to differentiate to derivatives of multiple progenitor cell types throughout the culture.
A method of treating a disease, disorder, or condition in a human comprising:
- administering a pharmacologically effective dose of human placental mesenchymal stem cells to the human.
A method for producing human placental mesenchymal stem cells comprising:
- digesting enzymatically placental villous tissue with trypsin and DNase;
- cultivating digested microvilli with 100% fetal bovine serum (FBS) followed by Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum and antibiotic-antimycotic solution;
- growing stromal cell columns;
- and
- sub-culturing placental mesenchymal stem cells.
Dependencies are inferred from the claim text.